| 11. | The constructed TaqMan PCR method was primarily used to mosquito samples test for JEV. Results Mg? 2+ was optimized at 5 mmol/L, ratio of forward and reverse primers at 4∶1. Test showed that the primers and probe were highly conservative and specific. The low detecting limit was 10 PFU/ml. |
| 结果Mg2+优化浓度为5mmolL,上下游引物浓度比例为4∶1,引物探针具有良好的保守性和特异性,灵敏度为10PFUml,稳定性分析表明,同一样品Ct值的重复检测5次,变异系数均小于5%; |
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| 12. | Double side probe plates:Test pads can bespread on both sides of PCB for convenient PCB layout. |
| 上下针板结构：测试点可双面选点，方便电路板布线设计。 |
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